Gap Junctions in Arthritis
Summary
Factors Involved in Osteoarthritis
Generic Synovial Joint
Organization of Synovium
Hypothetical Role of Membrane Potential (Vm) in Regulation of Synovial Cells
Why Vm?
Nystatin Patch-Clamp
Initial Measurements Cell Membrane Potential
Effect of Cytokines on Current-Voltage Curves
Mechanism of IL-1ß Effect
Discovery of Gap Junctions
Current Through Gap Junctions
Organization of Gap Junctions
Mammalian Connexins
Connexon Complexity
Relation between the Time Constant for Current Decay in HIG-82 Cells and Aggregate Size in Culture
Transient Current Response of a Synovial Lining Cell in Tissue
Western Blot Analysis
Immunohistochemical Detection in Cultured Human Synovial Cells
Immunohistochemical Detection in Cryosections of Human Synovial Tissue
Dye Transfer Through Gap Junctions
Gap Junctions in Human Synovium and between HIG-82 Cells
Gap-Junction Plaques in Normal and Arthritic Human Synovia
Metalloproteinase Assay
Protease Activity of IL-1ß TreatedSynovial Cells
Effect of IL-1ß on Protease Activity
Effect of 18a-glycyrrhetinic acid (GR) on Protease Production by Synovial Cells in the Presence of IL-1ß
Effect of Octanol on Protease Production by Synovial Cells in the Presence of IL-1ß
Effect of Brief Application ofGap Junction Inhibitors on Protease Production
Slide 32
Summary (Know)
Summary (Think)
Acknowledgments
Author: Dr. Andrew A. Marino
Email: amarino@lsuhsc.edu
Home Page: http://ortho.sh.lsuhsc.edu/Marino/
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